新功能、新界面、新体验,扫描即可下载生物谷APP!
首页 » 生物研究 » AJP:GI & Liver:利用人类细胞开发出功能化的工程化小肠组织

AJP:GI & Liver:利用人类细胞开发出功能化的工程化小肠组织

来源:生物谷 2015-01-13 10:03

2015年1月13日 讯 /生物谷BIOON/ --近日,一项刊登于国际杂志the American Journal of Physiology:GI & Liver上的研究报告中,来自洛杉矶儿童医院的研究人员通过研究表明,他们可以利用人类细胞来复制人类功能性小肠的关键部分,从而制造出人工组织工程化的小肠组织(Tissue-engineered small intestine ,TESI),这种人工化小肠组织包含着重要的粘膜及支持结构,同时也可以吸收糖类等物质,甚至也可以吸收一些小型或者超结构分子(细胞连接物),相关研究或可为开发再生医学疗法来治疗人类疾病提供思路和希望。

利用小肠干细胞开发出的人工组织工程化的小肠组织(TESI)或可有效治疗短肠综合征(SBS),SBS是肠衰竭的主要原因,尤其是对于早产儿及患有先天性肠道畸形的新生儿非常危险; Tracy C. Grikscheit博士表示,TESI或将有一天成为一种标准化的疗法来进行肠道移植,并且可以缓解当前面临移植组织短缺的现状。

本文研究旨在帮助改善患虚弱疾病的年轻患者的症状,包括那些早产而且患坏死性小肠结肠炎的新生儿,坏死性小肠结肠炎是一种危及生命的严重小肠疾病;如果小肠的长度不够,婴儿就会依赖静脉来进食,但这种方法价格非常昂贵而且往往会引发肝损伤。研究者表明,TESI可以植入免疫缺陷症的小鼠机体中,早在2011年的初期研究中,研究人员便仅在小肠组织中鉴别出了基本的组分。

而本文研究中,研究人员通过研究证实,小鼠的TESI和来自人类细胞的TESI高度相似,而且这两者都包含有重要的结构组分,比如可以分化再生为肠道组织的干细胞和祖细胞,而且工程化组织中的细胞对于健康人群的肠道发挥功能非常必要。

最后研究者Grikscheit表明,本文研究显示,我们可以开发出组织工程化的小肠组织,其相比其它的干细胞及祖细胞模型要更为复杂一些,而且可以用于进行肠道再生及相关疾病的研究,阐明这种工程组织化的肠道组织的功能或许具有里程碑的意义,其将来有望一天帮助治疗患肠道功能衰竭的患者。(生物谷Bioon.com)

本文系生物谷原创编译整理,欢迎转载!转载请注明来源并附原文链接。谢谢!

Human and Mouse Tissue-Engineered Small Intestine Both Demonstrate Digestive And Absorptive Function.

Christa Nicole Grant , Garcia Mojica Salvador , Frederic G Sala , Jeffrey Ryan Hill , Daniel E Levin , Allison L Speer , Erik R Barthel , Hiroyuki Shimada , Nicholas C. Zachos , Tracy C. Grikscheit

Objective Short bowel syndrome (SBS) is a devastating condition in which insufficient small intestinal surface area results in malnutrition and dependence on intravenous parenteral nutrition. There is an increasing incidence of SBS, particularly in premature babies and newborns with congenital intestinal anomalies. Tissue-engineered small intestine (TESI) offers a therapeutic alternative to the current standard treatment, intestinal transplantation, and has the potential to solve its biggest challenges: donor shortage and life-long immunosuppression. We have previously demonstrated that TESI can be generated from mouse and human small intestine and histologically replicates key components of native intestine. We hypothesized that TESI also recapitulates native small intestine function. Design Organoid units were generated from mouse or human donor intestine and implanted into genetically identical or immunodeficient host mice. After four weeks, TESI was harvested and either fixed and paraffin embedded, or immediately subjected to assays to illustrate function. Results We demonstrated that both mouse and human tissue-engineered small intestine grew into an appropriately polarized sphere of intact epithelium facing a lumen, contiguous with supporting mesenchyme, muscle, and stem/progenitor cells. The epithelium demonstrated major ultrastructural components including tight junctions and microvilli, transporters, and functional brush border and digestive enzymes. Conclusions This study demonstrates that tissue-engineered small intestine possesses a well-differentiated epithelium with intact ion transporters/channels, functional brush border enzymes, and similar ultrastructural components to native tissue including progenitor cells whether derived from mouse or human cells.

温馨提示:87%用户都在生物谷APP上阅读,扫描立刻下载! 天天精彩!


相关标签

最新会议 培训班 期刊库