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PNAS:科学家开发出修复严重组织损伤的新技术

来源:生物谷 2014-04-13 00:33

2014年4月13日 讯 /生物谷BIOON/ --近日,来自以色列理工学院(Technion-Israel Institute of Technology)的研究人员通过研究开发了一种修复严重组织损伤的新技术,这将可以加速恢复大面积软组织创伤患者的病情,并且减少疤痕和毁容的风险;通过对肌瓣进行生物工程操作,研究者就可以开发出一种新型组织,其在日后就可以连同病人血液一起移植到机体受损的部位。相关研究成果刊登于国际杂志PNAS上。

文章中,研究者Shulamit Levenberg教授表示,我们利用多种细胞和结缔组织就可以将肌瓣“焊接”起来,随后通过重建小鼠的腹壁结构来检测肌瓣的功能。在过去要进行大面积软组织缺失的重建非常困难,而如今科学家们利用支架和合成材料就可以对这些软组织缺失进行成功重建。

Levenberg表示,如今我们开始设计并且评估这种可生成血管的肌瓣的功能,合适的血管生成对于将肌瓣整合入宿主机体非常必要。这项研究就为组织特异性的细胞提供了一定证据,比如肌细胞、内皮细胞和成纤维细胞,其对于进行肌瓣的生物工程操作非常关键。

将肌瓣植入检测小鼠机体一周内,肌瓣就可以形成有活性的血管,而且肌瓣可以紧密吸附周围的组织上,这也就表明肌瓣具有支撑腹腔内脏的机械强度;这项研究对于医生们进行临床研究非常重要,未来临床医生们就可以利用肌瓣来修复个体因意外创伤导致的组织缺失。(生物谷Bioon.com)

doi:10.1073/pnas.1402679111
An engineered muscle flap for reconstruction of large soft tissue defects

Yulia Shandalova,1, Dana Egozib,c,1, Jacob Kofflera,d, Dekel Dado-Rosenfelda, David Ben-Shimolb, Alina Freimana,d, Erez Shora, Aviva Kabalaa, and Shulamit Levenberga,2

Large soft tissue defects involve significant tissue loss, requiring surgical reconstruction. Autologous flaps are occasionally scant, demand prolonged transfer surgery, and induce donor site morbidity. The present work set out to fabricate an engineered muscle flap bearing its own functional vascular pedicle for repair of a large soft tissue defect in mice. Full-thickness abdominal wall defect was reconstructed using this engineered vascular muscle flap. A 3D engineered tissue constructed of a porous, biodegradable polymer scaffold embedded with endothelial cells, fibroblasts, and/or myoblasts was cultured in vitro and then implanted around the femoral artery and veins before being transferred, as an axial flap, with its vascular pedicle to reconstruct a full-thickness abdominal wall defect in the same mouse. Within 1 wk of implantation, scaffolds showed extensive functional vascular density and perfusion and anastomosis with host vessels. At 1 wk posttransfer, the engineered muscle flaps were highly vascularized, were well-integrated within the surrounding tissue, and featured sufficient mechanical strength to support the abdominal viscera. Thus, the described engineered muscle flap, equipped with an autologous vascular pedicle, constitutes an effective tool for reconstruction of large defects, thereby circumventing the need for both harvesting autologous flaps and postoperative scarification.

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