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Cancer Res:利用前哨淋巴结T细胞表达CD30预测黑色瘤复发

来源:生物谷 2014-01-06 21:34

2014年1月6日讯 /生物谷BIOON/--前哨淋巴结中T细胞的CD30阳性表达的黑色素瘤患者更可能在五年内病情会进一步恶化,根据发表在Cancer Research杂志上的一项研究证实。使用基因图谱研究,Monica Rodolfo博士等人发现,前哨淋巴结中包含的信息有助于预测黑色素瘤患者是否将会是侵略性癌症。

虽然这项研究涉及到相对少数患者,但它提供了验证性的原则,即免疫系统是参与控制肿瘤生长的关键。Rodolfo解释:我们发现阳性表达CD30的T细胞较为频繁的存在侵袭性黑色素瘤患者的前哨淋巴结中,此外,这类T细胞在晚期患者的血液中也存在。

新研究的临床意义是明确的:有助寻找一种方法来避免对那些不太可能复发的病人开展进一步的手术治疗和深切治疗。研究结果也提示前哨淋巴结的基因研究可作为一个可靠的工具来开展个性化治疗。

Rodolfo和他的同事设计了一个探索性的研究,调查侵袭性疾病不同阶段的42名黑色素瘤患者的前哨淋巴结,并进行基因分析。他们的目的是确定一个分子“签名”,可以预测哪些患者肿瘤复发是高风险。

他们分析了肿瘤复发患者或手术切除原发肿瘤后五年没有复发的患者前哨淋巴结中免疫细胞称为淋巴细胞,包括T细胞。此外,研究人员收集25例3期和4期黑色素瘤患者血液样本和年龄和性别匹配的健康供者血液,并进行了比较。

他们发现,黑色素瘤复发以及晚期患者的前哨淋巴结中T细胞携带标记CD30表达上调。Rodolfo说:我们推测,CD30可能成为一个新的目标,旨在恢复有效的抗肿瘤免疫反应。(生物谷Bioon.com)

 

Transcriptional Profiling of Melanoma Sentinel Nodes Identify Patients with Poor Outcome and Reveal an Association of CD30+ T Lymphocytes with Progression

Viviana Vallacchi, et al.

Sentinel lymph nodes set the stance of the immune system to a localized tumor and are often the first site to be colonized by neoplastic cells that metastasize. To investigate how the presence of neoplastic cells in sentinel lymph nodes may trigger pathways associated with metastatic progression, we analyzed the transcriptional profiles of archival sentinel node biopsy specimens obtained from melanoma patients. Biopsies from positive nodes were selected for comparable tumor infiltration, presence or absence of further regional node metastases, and relapse at 5-year follow-up. Unsupervised analysis of gene expression profiles revealed immune response to be a major gene ontogeny represented. Among genes upregulated in patients with progressing disease, the TNF receptor family member CD30/TNFRSF8 was confirmed in biopsy specimens from an independent group of patients. Immunohistochemical analysis revealed higher numbers of CD30+ lymphocytes in nodes from progressing patients compared with nonprogressing patients. Phenotypic profiling demonstrated that CD30+ lymphocytes comprised a broad population of suppressive or exhausted immune cells, such as CD4+Foxp3+ or PD1+ subpopulations and CD4?CD8? T cells. CD30+ T lymphocytes were increased in peripheral blood lymphocytes of melanoma patients at advanced disease stages. Our findings reinforce the concept that sentinel nodes act as pivotal sites for determining progression patterns, revealing that the presence of CD30+ lymphocytes at those sites associate positively with melanoma progression. Cancer Res; 74(1); 130–40. ?2014 AACR.

 

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