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PLoS ONE:首次报道犊牛感染火鸡隐孢子虫

来源:中国疾控中心 2013-12-08 17:36

寄生虫病所重点实验室沈玉娟研究员作为通讯作者,与哈尔滨医科大学刘爱芹教授合作于2013年1月在《PLoS ONE》杂志上,发表“Distribution and Genetic Characterizations of Cryptosporidium spp. in Pre-Weaned Dairy Calves in Northeastern China’s Heilongjiang Province”。

该文报告了我国黑龙江省哈尔滨、大庆、牡丹江和齐齐哈尔地区断奶前犊牛隐孢子虫感染调查情况,总感染率为47.68%(72/151),分子鉴定为牛隐孢子虫47.22%(34/72),安氏隐孢子虫36.11%(26/72),赖氏隐孢子虫6.94%(5/72),火鸡隐孢子虫6.94%(5/72)和微小隐孢子虫2.78%(2/72)。其中微小隐孢子虫、安氏隐孢子虫和火鸡隐孢子虫,均为人兽共患隐孢子虫虫种,具有潜在的公共卫生危害。亚型分型:微小隐孢子虫亚型为IIdA19G1,火鸡隐孢子虫亚型为IIIeA22G2R1。具有我国地域流行特点,且国内外首次报道犊牛自然感染火鸡隐孢子虫。

火鸡隐孢子虫主要感染禽类,也是继人隐孢子虫和微小隐孢子虫后,第三大能感染人的隐孢子虫虫种。而犊牛作为火鸡隐孢子虫的储存宿主,需要开展更多系统的实验感染研究来证实;同时对人、动物进行进一步的分子流行病学研究,来阐明微小隐孢子虫和火鸡隐孢子虫等传播动力学关系。(生物谷Bioon.com)

生物谷推荐的英文摘要

PLoS ONE              DOI: 10.1371/journal.pone.0054857

Distribution and Genetic Characterizations of Cryptosporidium spp. in Pre-Weaned Dairy Calves in Northeastern China’s Heilongjiang Province

Weizhe Zhang equal contributor, Rongjun Wang equal contributor, Fengkun Yang, Longxian Zhang, Jianping Cao, Xiaoli Zhang, Hong Ling, Aiqin Liu mail, Yujuan Shen

Background

Cryptosporidium spp. are common parasites of humans and animals. Farm animals, especially pre-weaned calves, are considered to be one of main animal reservoir hosts of Cryptosporidium in the transmission of human cryptosporidiosis. The aim of this study was to determine the distribution and genotypes of Cryptosporidium spp. in pre-weaned calves using molecular tools and to assess zoonotic transmission and elucidate the public health significance in northeastern China.

Methodology/Principal Findings

A total of 151 fecal specimens from pre-weaned calves were collected in Heilongjiang Province and were screened for Cryptosporidium by PCR. The average prevalence of Cryptosporidium was 47.68% (72/151). Cryptosporidium spp. were characterized by DNA sequencing of the small subunit (SSU) rRNA gene and the 60-kDa glycoprotein (gp60) gene. Based on the SSU rRNA gene, five Cryptosporidium spp. were identified, including C. bovis (n = 34), C. andersoni (n = 26), C. ryanae (n = 5), C. meleagridis (n = 5) and C. parvum (n = 2). The SSU rRNA nucleotide sequences were identical to each other, respectively, within C. ryanae, C. parvum, C. meleagridis and C. andersoni. Four types of C. bovis were found in the SSU rRNA gene, with two novel types. The gp60 gene was successfully sequenced in one C. parvum isolate and three C. meleagridis isolates, with IIdA19G1 for C. parvum and IIIeA22G2R1 for C. meleagridis.

Conclusion/Significance

Molecular analysis indicates that Cryptosporidium spp. are endemic in pre-weaned calves in Heilongjiang Province. The findings of C. parvum and C. meleagridis suggested the possibility of zoonotic transmission and public health significance. The transmission dynamics of C. parvum and C. meleagridis needed to be clarified by further molecular epidemiologic studies from humans and animals. Whether calves could act as the natural reservoirs of C. meleagridis needed to be confirmed by more systematic experimental infection studies.

 

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