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NRR:神经干细胞向神经元分化:第2周钾电流密度爆发式增加

来源:EurekAlert 2013-12-05 11:07

K+通道的电生理特性可作为衡量神经干细胞功能性分化的一项基本指标。一项关于“Two outward potassium current types are expressed during the neural differentiation of neural stem cells”的研究分离获得增殖活性较高的神经干细胞,并能诱导生成神经细胞,且细胞分化过程伴随钾电流的表达,特别是在细胞分化发育的第2周,出现不同于其他研究的钾电流密度爆发式增加。

作者认为,新生乳鼠海马神经干细胞在体外能被诱导分化为功能性神经元,这些分化的神经元能表达2种类型的外向K+ 电流,从而具有类似体内成熟神经元的基本活性。

相关研究成果发表于2013年10月28期《中国神经再生研究(英文版)》杂志上。(生物谷Bioon.com)

生物谷推荐的英文摘要

Neural Regeneration Research               doi:10.3969/j.issn.1673-5374.2013.28.008

Two outward potassium current types are expressed during the neural differentiation of neural stem cells

Ruiying Bai[1]    Guowei Gao[2]    Ying Xing[1]    Hong Xue[3]

The electrophysiological properties of potassium ion channels are regarded as a basic index for determining the functional differentiation of neural stem cells. In this study, neural stem cells from the hippocampus of newborn rats were induced to differentiate with neurotrophic growth factor, and the electrophysiological properties of the voltage-gated potassium ion channels were observed. Immunofluorescence staining showed that the rapidly proliferating neural stem cells formed spheres in vitro that expressed high levels of nestin. The differentiated neurons were shown to express neuron-specific enolase. Flow cytometric analysis revealed that the neural stem cells were actively dividing and the percentage of cells in the S + G2/M phase was high. However, the ratio of cells in the S + G2/M phase decreased obviously as differentiation proceeded. Whole-cellpatch-clamp re-cordings revealed apparent changes in potassium ion currents as the neurons differentiated. The potassium ion currents consisted of one transient outward potassium ion current and one delayed rectifier potassium ion current, which were blocked by 4-aminopyridine and tetraethylammonium, respectively. The experimental findings indicate that neural stem cells from newborn rat hippo-campus could be cultured and induced to differentiate into functional neurons under defined condi-tions in vitro. The differentiated neurons expressed two types of outward potassium ion currents similar to those of mature neurons in vivo.

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