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Nucleic Acids Res:脂滴新研究模式的建立及微生物能源的开发

来源:中国科学院生物物理研究所 2013-10-25 20:53

2013年10月,《Nucleic Acids Research》在线发表了我所刘平生研究员课题组产油菌的研究工作,题目为“Integrated omics study delineates the dynamics of lipid droplets in Rhodococcus opacus PD630”。

由于化石能源的逐渐枯竭,迫使生物燃料开发的迅猛发展。以微生物为基础的新型可再生清洁能源将可能引爆新一轮“工业革命”。美国能源部的研究报告指出,单位面积土地上微藻的产油量是地面植物的100倍。而为人类基因组做出巨大贡献的J. Craig Venter团队正在积极构建一个高效产油菌种,目的是要“发掘一个用之不竭的油田”。

浊红球菌Rhodococcus opacus PD630是一种革兰氏阳性菌。该菌株拥有强大的碳源转化能力,能够将各种碳源,比如自然界含量极高的纤维素和木质素转化成甘油三酯,以脂滴的形式储存起来,其含油量可高达菌体干重的87%,是目前被公认的发展生物能源最具潜力的候选菌种之一。

刘平生研究员自2008年回国后,逐步建立并完善了脂滴研究的平台,利用基因组学、蛋白质组学、转录组学、及系统生物学方法,在基因组所于军课题组的协助下,对PD630这株高效产油菌展开了系统性研究,首次测序并公布了该产油菌的全基因组信息、脂滴蛋白质组信息,和转录组学数据。并在此基础上进行了一些目标基因的研究。这些结果的发表,将有助于建立以原核生物为基础的新型脂滴研究模式,相较于复杂的真核细胞系统,不但更有利于促使脂滴生物学领域基本问题的解决,同时会大力推动微生物生物能源的开发。

该项工作得到科技部、国家自然科学基金委员会的资助。(生物谷Bioon.com)

生物谷推荐的英文摘要



Nucleic Acids Research  doi:10.1093/nar/gkt932

Integrated omics study delineates the dynamics of lipid droplets in Rhodococcus opacus PD630.
 
Chen Y, Ding Y, Yang L, Yu J, Liu G, Wang X, Zhang S, Yu D, Song L, Zhang H, Zhang C, Huo L, Huo C, Wang Y, Du Y, Zhang H, Zhang P, Na H, Xu S, Zhu Y, Xie Z, He T, Zhang Y, Wang G, Fan Z, Yang F, Liu H, Wang X, Zhang X, Zhang MQ, Li Y, Steinbüchel A, Fujimoto T, Cichello S, Yu J, Liu P.

Rhodococcus opacus strain PD630 (R. opacus PD630), is an oleaginous bacterium, and also is one of few prokaryotic organisms that contain lipid droplets (LDs). LD is an important organelle for lipid storage but also intercellular communication regarding energy metabolism, and yet is a poorly understood cellular organelle. To understand the dynamics of LD using a simple model organism, we conducted a series of comprehensive omics studies of R. opacus PD630 including complete genome, transcriptome and proteome analysis. The genome of R. opacus PD630 encodes 8947 genes that are significantly enriched in the lipid transport, synthesis and metabolic, indicating a super ability of carbon source biosynthesis and catabolism. The comparative transcriptome analysis from three culture conditions revealed the landscape of gene-altered expressions responsible for lipid accumulation. The LD proteomes further identified the proteins that mediate lipid synthesis, storage and other biological functions. Integrating these three omics uncovered 177 proteins that may be involved in lipid metabolism and LD dynamics. A LD structure-like protein LPD06283 was further verified to affect the LD morphology. Our omics studies provide not only a first integrated omics study of prokaryotic LD organelle, but also a systematic platform for facilitating further prokaryotic LD research and biofuel development.

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