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首页 » 纳米医学 » Analyt Chim Acta:开发出一种检测早期感染的光-纳米探针技术

Analyt Chim Acta:开发出一种检测早期感染的光-纳米探针技术

来源:生物谷 2013-06-23 15:16

2013年6月22日 讯 /生物谷BIOON/ --近日,刊登在国际杂志Analytica Chimica Acta上的一篇研究报告中,来自杜克大学的研究者通过研究开发出了一种新型技术,使用光和纳米探针来检测病人机体的感染情况(病人未表现出症状之前)。这种新型技术使用人的组织样品,可进行快速高效的检测。

文章中研究者开发出了一种纳米银颗粒,其可以坐落在特殊的分子标记物上,然后在个体感染的第一阶段通过渗透进入机体血管中,当光照射到样品上时,纳米颗粒就会吸附到分子标记物上,从而反映出不同的光学指纹。研究者Tuan Vo-Dinh表示,我们首次揭示了使用纳米探针可以检测来自人类组织样品的特殊遗传物质。

纳米探针使用可以结合表面增强拉曼散射技术(SERS),当激光照射在样品表面时,靶向分子就会震动然后散射成独特的光束,通常称为拉曼散射,但是一般来讲,拉曼效应极其微弱。当靶向分子和金属纳米颗粒或者纳米探针偶联时,拉曼效应就可以通过SERS效应来增强,通常可以增强超过一百万倍。

研究者Geoffrey Ginsburg表示,这项研究为将来开发新型设备检测多种感染性疾病提供了思路和帮助,而且这也可以帮助临床医生尽早得到诊断结果,以便及时对患者进行相应感染性疾病的治疗或者抗菌疗法的使用。(生物谷Bioon.com)

Surface-enhanced Raman scattering molecular sentinel nanoprobes for viral infection diagnostics

Hsin-Neng Wanga, b, Andrew M. Falesa, b, Aimee K. Zaasc, Christopher W. Woodsc, Thomas Burkec, Geoffrey S. Ginsburgb, c, Tuan Vo-Dinha, b, d, ,

In this paper, we describe a surface-enhanced Raman scattering (SERS)-based detection approach, referred to as “molecular sentinel” (MS) plasmonic nanoprobes, to detect an RNA target related to viral infection. The MS method is essentially a label-free technique incorporating the SERS effect modulation scheme associated with silver nanoparticles and Raman dye-labeled DNA hairpin probes. Hybridization with target sequences opens the hairpin and spatially separates the Raman label from the silver surface thus reducing the SERS signal of the label. Herein, we have developed a MS nanoprobe to detect the human radical S-adenosyl methionine domain containing 2 (RSAD2) RNA target as a model system for method demonstration. The human RSAD2 gene has recently emerged as a novel host-response biomarker for diagnosis of respiratory infections. Our results showed that the RSAD2 MS nanoprobes exhibits high specificity and can detect as low as 1 nM target sequences. With the use of a portable Raman spectrometer and total RNA samples, we have also demonstrated for the first time the potential of the MS nanoprobe technology for detection of host-response RNA biomarkers for infectious disease diagnostics.

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