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NRR:睫状神经营养因子影响神经祖细胞分化途径

来源:EurekAlert!中文 2013-03-06 11:10

自发性分化是神经干细胞的特性,而之前的很多关于神经干细胞定向分化的研究都没有考虑到自发性分化对实验结果的影响,因此,自发性分化成为研究神经干细胞生物学特点及临床应用必须面对的问题。

睫状神经营养因子是迄今惟一发现的可以促进成体大鼠海马神经祖细胞向胶质和神经元方向分化的神经营养因子,这与自发性分化的结果相似。但是,睫状神经营养因子是否参与了神经干细胞自发性分化过程?

 睫状神经营养因子诱导后,74%的大鼠海马神经祖细胞表达Tuj1

2013年2月第4期出版的《中国神经再生研究(英文版)》(NEURAL REGENERATION RESEARCH)杂志中一项关于“Influence of endogenous ciliary neurotrophic factor on neural differentiation of adult rat hippocampal progenitors”的研究显示,培养的海马神经祖细胞表达内源性睫状神经营养因子,睫状神经营养因子抗体能够减缓海马神经祖细胞向神经元和神经胶质细胞的分化。因此作者认为,内源性睫状神经营养因子介导海马神经祖细胞的自发分化。(生物谷Bioon.com)

链接阅读:

睫状神经营养因子及受体的研究进展

Influence of endogenous ciliary neurotrophic factor on neural differentiation of adult rat hippocampal progenitors

Jun Ding, Zhili He, Juan Ruan, Ying Liu, Chengxin Gong, Shenggang Sun,  Honghui Chen.

Ciliary neurotrophic factor is the only known neurotrophic factor that can promote differentiation of hippocampal neural progenitor cells to glial cells and neurons in adult rats. This process is similar to spontaneous differentiation. Therefore, ciliary neurotrophic factor may be involved in spontaneous differentiation of neural stem cells. To verify this hypothesis, the present study isolated neural progenitor cells from adult male rats and cultured them in vitro. Results showed that when neural progenitor cells were cultured in the absence of mitogen fibroblast growth factor-2 or epidermal growth factor, they underwent spontaneous differentiation into neurons and glial cells. Western blot and immunocytochemical staining showed that exogenous ciliary neurotrophic factor strongly induced adult hippocampal progenitor cells to differentiate into neurons and glial cells. Moreover, passage 4 adult hippocampal progenitor cells expressed high levels of endogenous ciliary neurotrophic factor, and a neutralizing antibody against ciliary neurotrophic factor prevented the spontaneous neuronal and glial differentiation of adult hippocampal progenitor cells. These results suggest that the spontaneous differentiation of adult hippocampal progenitor cells is mediated partially by endogenous ciliary neurotrophic factor.

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