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ACS Nano:开发出快速检测疾病发生的DNA纳米探针技术

来源:生物谷 2012-10-10 22:56

2012年10月10日 讯 /生物谷BIOON/ --许多犯罪分子作案都会留下线索,类似地,人类的许多疾病,比如癌症,也会留在某些遗传线索。用于检测疾病发病中相关DNA的工具,如miRNAs,其由于花费高检测速度慢,逐渐变得并不适用。

近日,来自哥本哈根大学的研究者发明出了一种新型方法可以更加快速地检测出相关的人类疾病。相关研究成果刊登于国际杂志ACS Nano上。

研究者开发出了一种DNA传感器,其可以将遗传物质结合至发光分子上,这种发光分子仅仅在特异性靶点存在的情况下才会发光。

单一的DNA链是由碱基对构成的,当两股DNA链形成双链DNA的时候,其通过互补碱基对的相互结合吸附来完成双链形成。类似地,研究者开发出了这种新型技术,他们检测了携带8中不同类型遗传物质的新型银纳米DNA探针,其可以对包括癌症在内的许多类型疾病中涉及的miRNAs进行检测,当前传统的检测方法需要48小时,而这种技术仅仅需要最多6小时。研究者希望他们的研究可以早日应用于检测疾病的发生。(生物谷Bioon.com)

编译自:Glowing DNA Invention Points Towards High Speed Disease Detection

Design Aspects of Bright Red Emissive Silver Nanoclusters/DNA Probes for MicroRNA Detection

Pratik Shah †, Andreas Rørvig-Lund ‡, Samir Ben Chaabane †, Peter Waaben Thulstrup §, Henrik Grum Kjaergaard ‡, Eduard Fron , Johan Hofkens , Seong Wook Yang †*, and Tom Vosch ‡*

The influence of the nucleic acid secondary structure on the fast (1 h) formation of bright red emissive silver nanoclusters (AgNCs) in a DNA sequence (DNA-12nt-RED-160), designed for the detection of a microRNA sequence (RNA-miR160), was investigated. The findings show that especially the propensity for mismatch self-dimer formation of the DNA probes can be a good indicator for the creation and stabilization of red emissive AgNCs. Also, the role of the thermal stability of the secondary DNA structures (mismatch self-dimer and hairpin monomers) and the observed AgNC red emission intensity were investigated. These findings can form the basis for a rationale to design new red emissive AgNC-based probes. As an example, a bright red emissive AgNC-based DNA probe was designed for RNA-miR172 detection. The latter opens the possibility to create a variety of AgNC-based DNA probes for the specific detection of plant and animal miRNAs.

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