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首页 » 华人研究 » J Virol:蓝柯等卡波氏肉瘤相关疱疹病毒颗粒研究获进展

J Virol:蓝柯等卡波氏肉瘤相关疱疹病毒颗粒研究获进展

来源:中科院巴斯德所 2012-09-20 18:59

长期以来,人们一直认为病毒颗粒中只含有一种作为遗传物质的核酸。然而,最近十年左右的研究表明,许多DNA病毒颗粒中除含有作为遗传物质的DNA之外,还存在着许多mRNA分子。但是DNA病毒颗粒中具体RNA分子的构成仍然很不清楚。

中科院上海巴斯德所蓝柯研究组通过深度测序和定量逆转录PCR实验(图1),发现在纯化的KSHV病毒颗粒中含有部分病毒编码miRNA和大量宿主编码的miRNA。除了miRNA以外,在纯化的KSHV病毒颗粒RNA中还检测到大量miRNA来源usRNA以及U2 snRNA来源的usRNA。

原位杂交-电镜(ISH-EM)实验(图2)检测到miRNA与病毒颗粒共定位,直接证实miRNA被包装进入KSHV病毒颗粒中。进一步实验表明这些miRNA能够随着病毒感染进入宿主细胞,并且具有抑制靶基因表达的生物学活性。这一发现拓展了对DNA病毒尤其是疱疹病毒颗粒结构成分的认识,为开发抗疱疹病毒药物提供了可能的干预靶点。

国际知名学术期刊Journal of Virology 9月12日在线发表了这一最新研究成果。

该项工作在博士研究生林先志在蓝柯研究员指导下完成的,并得到中科院生物物理研究所邓红雨研究员的大力协助。该工作得到国家973计划、国家自然科学基金和中科院百人计划的经费支持。(生物谷Bioon.com)

miRNAs and usRNAs Discovered in Kaposi's Sarcoma-associated Herpesvirus Virions

Xianzhi Lin, Xiaofan Li, Deguang Liang and Ke Lan#

It is widely held that any given virus uses only one type of nucleic acids for genetic information storage. However, this consensus has been challenged a little bit by several recent studies showing that many RNA species are present within a range of DNA viruses that include Kaposi's sarcoma-associated herpesvirus (KSHV). RNAs extracted from purified DNA virus particles exhibit great diversity in terms of length, abundance, temporal expression, cellular localization, and coding capacity during viral infection. In addition to known RNA species, the current study showed that small regulatory RNAs were present in KSHV virion. A large number of viral and cellular miRNAs, as well as unusual small RNAs (usRNAs), were detected in KSHV virion by using deep sequencing. Both viral and host miRNAs detected in small RNAs extracted from KSHV virions were further shown to co-localize with KSHV virion directly by in situ hybridization (ISH)-electron microscopy (EM) (ISH-EM). Some of these miRNAs were differentially present in the host cells and KSHV virions, suggesting that they are not randomly present in KSHV virions. The virional miRNAs could be transported into host cells and they are biologically functional during de novo viral infection. Our study revealed miRNAs and usRNAs as a novel group of component in KSHV virion.

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