打开APP

PLoS Negl Trop Dis:首次完成对导致疟疾的间日疟原虫基因组测序

  1. 基因组
  2. 疟疾
  3. 等位基因
  4. 间日疟原虫

来源:生物谷 2012-11-19 08:25

2012年9月8日 讯 /生物谷BIOON/ --来自美国凯斯西储大学(Case Western Reserve University)和克利夫兰诊所勒纳研究所(Cleveland Clinic Lerner Research Institute )的研究人员发现导致最常见疟疾类型的疟原虫---间日疟原虫(Plasmodium vivax)---拥有相同的基因变异...

2012年9月8日 讯 /生物谷BIOON/ --来自美国凯斯西储大学(Case Western Reserve University)和克利夫兰诊所勒纳研究所(Cleveland Clinic Lerner Research Institute )的研究人员发现导致最常见疟疾类型的疟原虫---间日疟原虫(Plasmodium vivax)---拥有相同的基因变异,即便这种疟原虫是从不同大陆分离到的。这项发现让人们担心,抵抗现存药物的突变能够在全世界扩散,从而使得全球根除疟疾的努力更加困难。

包括克利夫兰诊所勒纳研究所研究员David Serre和Peter Zimmerman、Didier Menard(柬埔寨巴斯德研究所)和Arsene Ratsimbasoa(马达加斯加国家疟疾控制项目)在内的研究人员是第一次对覆盖范围内病人身上提取的间日疟原虫基因组进行测序,以便证实全基因组范围的DNA序列变异。这种有机体的基因组含有它的所有可遗传性的信息。相关研究结果在9月6日刊登在PLoS Neglected Tropical Diseases期刊上。

科学家首先吃惊地发现在来自马达加斯加、柬埔寨和南美洲的人们的样品中,很少有不同位点特异性的基因变异。

在这项研究中,研究人员在首先除去白细胞之后,对来自2名马达加斯加病人和3名柬埔寨病人体内的血液样品中的间日疟原虫进行基因组测序。作为对比,他们在遭受在南美洲发现的人间日疟原虫菌株感染的一只猴子体内,获取间日疟原虫,并对这种疟原虫进行基因组测序。这些结果证实这6种样品广泛地拥有相同的等位基因。

如今,研究人员鉴定出8万个遗传标记,这些标记能够被用来寻找它们与耐药性疟疾之间的关联性。

研究人员将利用这些数据进行遗传进化研究来了解间日疟原虫是如何起源的,它是如何扩散的,以及不同的菌株在地理上是如何分布的。同时他们也将研究这种不断进化的疟原虫的感染机制。(生物谷Bioon.com)

Whole Genome Sequencing of Field Isolates Provides Robust Characterization of Genetic Diversity in Plasmodium vivax

Ernest R. Chan1#, Didier Menard2#*, Peter H. David3, Arsène Ratsimbasoa4, Saorin Kim2, Pheaktra Chim2, Catherine Do2, Benoit Witkowski2, Odile Mercereau-Puijalon3*, Peter A. Zimmerman5¶*, David Serre

Background An estimated 2.85 billion people live at risk of Plasmodium vivax transmission. In endemic countries vivax malaria causes significant morbidity and its mortality is becoming more widely appreciated, drug-resistant strains are increasing in prevalence, and an increasing number of reports indicate that P. vivax is capable of breaking through the Duffy-negative barrier long considered to confer resistance to blood stage infection. Absence of robust in vitro propagation limits our understanding of fundamental aspects of the parasite's biology, including the determinants of its dormant hypnozoite phase, its virulence and drug susceptibility, and the molecular mechanisms underlying red blood cell invasion. Methodology/Principal Findings Here, we report results from whole genome sequencing of five P. vivax isolates obtained from Malagasy and Cambodian patients, and of the monkey-adapted Belem strain. We obtained an average 70–400 X coverage of each genome, resulting in more than 93% of the Sal I reference sequence covered by 20 reads or more. Our study identifies more than 80,000 SNPs distributed throughout the genome which will allow designing association studies and population surveys. Analysis of the genome-wide genetic diversity in P. vivax also reveals considerable allele sharing among isolates from different continents. This observation could be consistent with a high level of gene flow among parasite strains distributed throughout the world. Conclusions Our study shows that it is feasible to perform whole genome sequencing of P. vivax field isolates and rigorously characterize the genetic diversity of this parasite. The catalogue of polymorphisms generated here will enable large-scale genotyping studies and contribute to a better understanding of P. vivax traits such as drug resistance or erythrocyte invasion, partially circumventing the lack of laboratory culture that has hampered vivax research for years.

版权声明 本网站所有注明“来源:生物谷”或“来源:bioon”的文字、图片和音视频资料,版权均属于生物谷网站所有。非经授权,任何媒体、网站或个人不得转载,否则将追究法律责任。取得书面授权转载时,须注明“来源:生物谷”。其它来源的文章系转载文章,本网所有转载文章系出于传递更多信息之目的,转载内容不代表本站立场。不希望被转载的媒体或个人可与我们联系,我们将立即进行删除处理。

87%用户都在用生物谷APP 随时阅读、评论、分享交流 请扫描二维码下载->