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中美合作完成中国仓鼠卵巢细胞系基因组测序

来源:新华社 2011-08-03 14:52

中外研究人员7月31日在英国著名学术杂志《自然—生物技术》(Nature Biotechnology)网络版上说,他们已完成对中国仓鼠卵巢细胞系的基因组测序。

中国仓鼠卵巢细胞系对生物制药意义重大。全球药物蛋白每年的市场销售额约为990亿美元,其中至少70%的药物蛋白来源于中国仓鼠卵巢细胞系。

中国深圳华大基因研究院与美国GT生命科学公司的研究人员在论文中说,他们采用新一代测序技术对中国仓鼠卵巢K1细胞系进行测序、组装,共预测了2.4万多个基因,并对预测的基因进行了功能注释。

该研究报告的第一作者徐讯说,中国仓鼠卵巢细胞系基因组测序的完成将有助于从基因水平进一步了解其细胞的功能,从而促进对该细胞系的优化应用,降低生产成本。

华大基因研究院副院长王俊认为,中国仓鼠卵巢细胞系基因组测序的完成为今后系统且高效地研究更多细胞系提供了基础,这是将基因组学应用于生物医药生产的重要一步。(生物谷 Bioon.com)

The genomic sequence of the Chinese hamster ovary (CHO)-K1 cell line

Xun Xu; Harish Nagarajan; Nathan E Lewis; Shengkai Pan; Zhiming Cai; Xin Liu; Wenbin Chen; Min Xie; Wenliang Wang; Stephanie Hammond; Mikael R Andersen; Norma Neff; Benedetto Passarelli; Winston Koh; H Christina Fan; Jianbin Wang; Yaoting Gui; Kelvin H Lee; Michael J Betenbaugh; Stephen R Quake; Iman Famili; Bernhard O Palsson; Jun Wang

Chinese hamster ovary (CHO)–derived cell lines are the preferred host cells for the production of therapeutic proteins. Here we present a draft genomic sequence of the CHO-K1 ancestral cell line. The assembly comprises 2.45 Gb of genomic sequence, with 24,383 predicted genes. We associate most of the assembled scaffolds with 21 chromosomes isolated by microfluidics to identify chromosomal locations of genes. Furthermore, we investigate genes involved in glycosylation, which affect therapeutic protein quality, and viral susceptibility genes, which are relevant to cell engineering and regulatory concerns. Homologs of most human glycosylation-associated genes are present in the CHO-K1 genome, although 141 of these homologs are not expressed under exponential growth conditions. Many important viral entry genes are also present in the genome but not expressed, which may explain the unusual viral resistance property of CHO cell lines. We discuss how the availability of this genome sequence may facilitate genome-scale science for the optimization of biopharmaceutical protein production.

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