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首页 » Transgenic Research:甘薯SRD1启动子可在拟南芥、胡萝卜和马铃薯中高效表达

Transgenic Research:甘薯SRD1启动子可在拟南芥、胡萝卜和马铃薯中高效表达

来源:国际农业生物技术周报 2011-07-13 10:14

植物淀粉贮藏器官是重要的农产品,也是分子农业中重组蛋白研究的重要靶向器官。为了充分挖掘蛋白在贮藏器官的表达能力,韩国高丽大学的Seol  Ah  No及其同事从甘薯中分离出SRD1启动子,并考察了该启动子在转基因拟南芥、胡萝卜和马铃薯中的活性。

研究表明,SRD1启动子分别在转基因拟南芥和马铃薯的根和块茎中表达,在转基因胡萝卜中,SRD1表现出主根特异性表达,并且主根的直径有所增加,这表明启动子活性提高。

研究人员最后得出结论,认为SRD1启动子可在地下贮藏器官中高效表达。 (生物谷Bioon.com)

生物谷推荐原文出处:

Transgenic Research    DOI: 10.1007/s11248-011-9528-4

A sweetpotato SRD1 promoter confers strong root-, taproot-, and tuber-specific expression in Arabidopsis, carrot, and potato

Seol Ah Noh, Haeng-Soon Lee, Gyung Hye Huh, Mi-Joung Oh, Kyung-Hee Paek, Jeong Sheop Shin and Jung Myung Bae

Harvestable, starch-storing organs of plants, such as fleshy taproots and tubers, are important agronomic products that are also suitable target organs for use in the molecular farming of recombinant proteins due to their strong sink strength. To exploit a promoter directing strong expression restricted to these storage organs, we isolated the promoter region (3.0 kb) of SRD1 from sweetpotato (Ipomoea batatas cv. ‘White Star’) and characterized its activity in transgenic Arabidopsis, carrot, and potato using the β-glucuronidase (GUS) gene (uidA) as a reporter gene. The SRD1 promoter conferred root-specific expression in transgenic Arabidopsis, with SRD1 promoter activity increasing in response to exogenous IAA. A time-course study of the effect of IAA (50 μM) revealed a maximum increase in SRD1 promoter activity at 24 h post-treatment initiation. A serial 5′ deletion analysis of the SRD1 promoter identified regions related to IAA-inducible expression as well as regions containing positive and negative elements, respectively, controlling the expression level. In transgenic carrot, the SRD1 promoter mediated strong taproot-specific expression, as evidenced by GUS staining being strong in almost the entire taproot, including secondary phloem, secondary xylem and vascular cambium. The activity of the SRD1 promoter gradually increased with increasing diameter of the taproot in the transgenic carrot and was 10.71-fold higher than that of the CaMV35S promoter. The SRD1 promoter also directed strong tuber-specific expression in transgenic potato. Taken together, these results demonstrate that the SRD1 promoter directs strong expression restricted to the underground storage organs, such as fleshy taproots and tubers, as well as fibrous root tissues.

 

 

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